We use none foot print, feeder-free in vitro reprogramming method to convertsomatic cells into patient specificiPSCs. IxCell?–iPSCs can be utilized as a reliable, unlimited, and ethically unbiased source for simulating personalized disease. Because of their pluripotency, IxCell?–iPSCs can be differentiated into various types of tissue cells, such as Cardiomyocytes, Hepatocytes, and Neurons, etc. IxCell?–iPSCs,carrying the phenotype of human disease,might also be useful for disease diagnosis. IxCell can provide IxCell?–iPSCs as the best in vitro cell model for disease research, diagnosis and personalized cell therapy evaluation.
Identification of IxCell?–iPSCs:
Figure 1 Phase Contrast
Figure 2 A-D Immunofluorescence staining of pluripotent markers. A-D：Nanog，Oct4， Sox2 , SSEA4.
Figure 3 In vitro EB differentiation . A-C: ectoderm(β-tubulin III), mesoderm（SMA）and endoderm（AFP）
Figure 4 Flow Cytometry analysis of pluripotent markers . A-C: Nanog, Oct4 and SSEA4
Figure 5 Karyotype analysis
Figure 6 Clearance test of episomal vector